Glucometer (glucose meter) is point of care testing device used for measuring glucose in fresh capillary whole blood. This system is plasma — calibrated to allow easy comparison of results with laboratory methods. It could be used for self— monitoring of blood glucose level by diabetes patients.
The enzyme glucose oxidase on the test strip reacts specifically with the blood glucose. The current generated is converted and displayed as blood glucose value
1. Insert the test strip: Remove test strip from the vial. Recap the vial immediately to prevent moisture from affecting the other strips. Insert the test strip.
2. Compare the code number: The meter will automatically turn ‘ON’ and display the code number. Make sure the code number on the display matches the code number on the test strip vial. If the code no does not match, code the meter correctly.
3. Applying blood sample: After 3 seconds of code confirmation blood icon will be displayed on the screen. Prick the area where you have decided to obtain the blood.
4. Touch your finger to the tip of Omnitest-plustest strip.
The measurement chamber of the test strip will draw automatically the blood of your finger. Your finger should remain still, until the confirmation window is completely filled and you hear the \”beep\” sound. The minimal sample volume is 1 uL.
Results in Seconds:
After \”beep\” sounds, the test will begin automatically, counting down the numbers from 5 to 1 on the display. Then the test result will be shown.
Blood glucose test results are displayed on the Omnitest-plustest meter as either milligrams of glucose per decilitre of blood (mg/dL). The Omnitest-plustest meter displays results between 10 –600 mg/dL.
2. Remove Tested Strip by Ejector:
Slide the ejector button forward to remove the test strip from the meter. The meter is turned ‘OFF’ with the removal of the test strip. Discard the used strip and lancet to a proper place.
It is a point of care testing device, which provides a quick and easy way to measure the blood glucose level.
It could be used for self-monitoring of blood glucose level by diabetes patients.
It should be used only for testing blood glucose and only with fresh capillary whole blood samples.
It should not be used for the diagnosis of diabetes or for the testing of new-borns (neonates).
Do not use this device for any purpose other than blood glucose test.
Instructions for Urine analysis by Dipsticks
Dipsticks (urine analysis reagent strips) are used for both qualitative and semi-quantitative urine analysis. These contain in-vitro reagent for diagnostics. (Dry chemistry tests). The urine parameters that can be tested for using dipsticks are: Leukocytes, nitrite, urobilinogen, protein, pH, blood, specific gravity, ketone bodies (acetoacetic acid), glucose & bilirubin. The results of the specific test parameters on the strips can be read visually as well as using instruments.
Principle of each parameter tested using dipstick
Glucose: Glucose oxidized by glucose oxidase catalyses the formation of gluconic acid and hydrogen peroxide. Hydrogen peroxide releases nascent [O] by the action of peroxidase. Nascent [O] oxidises potassium iodide which results in a color change. High concentration of ketones may give a false negative result.
Bilirubin: Direct bilirubin reacts with dichlorobenzone diazonium to produce azodyes in a strongly acid medium. Medicines that dye urine red can give false positive results and ascorbic acid may give false negative result.
Ketones: Acetoacetate produces violet color with sodium nitroprusside in alkaline medium. Highly pigmented urine or levodopa in urine may give false positive results.
Specific gravity: Electrolytes in the form of salt (MT) in urine reacts with poly methyl vinyl ether and malic acid which are weak acid (anionic) exchanger. The reaction produces hydrogen ion which reacts with the indicator and changes the color. This test detects specific gravity between 1.0 and 1.030. Proteinuria elevates the readings.
Blood: Hb (Heme) acts as peroxidase. It can causé release of nascent [O] using 1-1202 which oxidises the indicator and produces the color change subsequently.
Note: Certain oxidizing contaminants like hypochlorite or microbial peroxidase (in case of UTI) may lead t false positive results.
DU: The method of pH indicator is applied (pH detected in the range: 5.0 — 8.5).Different indicators change color at different pH.
Note: A highly alkaline urine may give false positive results.
Protein: pH indicator which is anionic reacts with the cationic protein, which produces a color change.
Urobilinogen: based on Ehrlich reaction. Paradimethyl amino benzaldehyde (in conjunction with a color enhancer) reacts with urobilinogen in a strongly acid medium to produce a reddish pink color. Detects amounts as low 3 umol/L.
Sample collection and specimen preparation
Collect fresh urine in a clean and dry container. Mix the sample properly before performing the test. Urine test should be performed within 2 hours of collection.
Note: Long term storage specimen may affect the test results of glucose, pH, nitrite & blood due to bacterial growth.
1. Immerse the reagent area of the strip in the urine specimen and take it up quickly.
2. Run the edge of the strip against the rim of the container to remove the excess urine. Excess sample can be removed by gently tapping over a filter paper.
3. Hold the strip up horizontally and compare the result on the strip with the color chart. Make note of the result. The strip should be read between 1-2 minutes after dipping as indicated in the color chart .Color changes beyond 2 minutes are of no diagnostic value.
Storage of strips:
1. Must be kept in original bottle
2. Do not use after expiry date
3. Every strip can be used only once
4. Cap the bottle immediately and tightly after taking out the strips
5. Strips should be stored at room temperature in a dry place
6. Do not touch the reagent area of the strip